دوره 27، شماره 4 - ( 9-1402 )
جلد 27 شماره 4 صفحات 210-203 |
برگشت به فهرست نسخه ها
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Yazdani S, Gaeini A, Koushkie Jahromi M, Hemmatinafar M. The Effect of High-intensity Interval Training and Moderate-intensity Continuous Training on Oxidative Stress Markers in Left Ventricular Tissue of Male Rats. Hormozgan Medical Journal 2023; 27 (4) :203-210
URL: http://hmj.hums.ac.ir/article-1-37-fa.html
URL: http://hmj.hums.ac.ir/article-1-37-fa.html
The Effect of High-intensity Interval Training and Moderate-intensity Continuous Training on Oxidative Stress Markers in Left Ventricular Tissue of Male Rats. مجله پزشکی هرمزگان. 1402; 27 (4) :203-210
چکیده: (255 مشاهده)
Objectives: The present study aimed to assess and compare the effects of high-intensity interval training and moderate-intensity continuous training on oxidative stress-related markers in the left ventricular tissue of the heart muscle in healthy male rats.
Methods: Fifteen male Wistar rats, aged 50-70 days, were randomly divided into 3 groups: Control group (CON), high-intensity interval training (HIIT), and moderate-intensity continuous training (MICT). After determining the maximum speed of VO2max, the training program of HIIT (six 2-min periods with 85%-90% VO2max and five 2-min with 50%-60% VO2max intensity) and MICT (same distance of running as HIIT with 70% VO2max intensity) groups were performed for 8 weeks, 5 days per week. Forty-eight hours after the last intervention session, the animals were anesthetized, and their heart tissue was removed and stored at -80°C. Then, the expression rates of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α), AMP-activated protein kinase (AMPK), and superoxide dismutase (SOD) genes were measured by real-time PCR, and the concentration of malondialdehyde (MDA) in left ventricular tissue of heart was measured by colorimetry method. One-way analysis of variance and Tukey post hoc test were used for data analysis at the significance level of P<0.05.
Results: There were significant differences between the groups’ expression rates of AMPK, PGC-1α, and SOD (P<0.05). The expression rates of AMPK and PGC-1α were higher in the MICT group than in the CON group (P<0.05). No significant difference was observed between HIIT and MICT groups regarding AMPK and PGC-1α (P>0.05). SOD expression was significantly higher in the HIIT group than CON and MICT groups (P<0.05). SOD expression was also higher in the MICT group compared to the CON group (P<0.05). MDA concentration was not significantly different between the studied groups (P>0.05).
Discussion: HIIT and MICT induce positive adaptations in the expression of AMPK, PGC-1α, and SOD in the heart tissue of healthy male rats. However, HIIT stimulates SOD gene expression more than MICT.
Methods: Fifteen male Wistar rats, aged 50-70 days, were randomly divided into 3 groups: Control group (CON), high-intensity interval training (HIIT), and moderate-intensity continuous training (MICT). After determining the maximum speed of VO2max, the training program of HIIT (six 2-min periods with 85%-90% VO2max and five 2-min with 50%-60% VO2max intensity) and MICT (same distance of running as HIIT with 70% VO2max intensity) groups were performed for 8 weeks, 5 days per week. Forty-eight hours after the last intervention session, the animals were anesthetized, and their heart tissue was removed and stored at -80°C. Then, the expression rates of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α), AMP-activated protein kinase (AMPK), and superoxide dismutase (SOD) genes were measured by real-time PCR, and the concentration of malondialdehyde (MDA) in left ventricular tissue of heart was measured by colorimetry method. One-way analysis of variance and Tukey post hoc test were used for data analysis at the significance level of P<0.05.
Results: There were significant differences between the groups’ expression rates of AMPK, PGC-1α, and SOD (P<0.05). The expression rates of AMPK and PGC-1α were higher in the MICT group than in the CON group (P<0.05). No significant difference was observed between HIIT and MICT groups regarding AMPK and PGC-1α (P>0.05). SOD expression was significantly higher in the HIIT group than CON and MICT groups (P<0.05). SOD expression was also higher in the MICT group compared to the CON group (P<0.05). MDA concentration was not significantly different between the studied groups (P>0.05).
Discussion: HIIT and MICT induce positive adaptations in the expression of AMPK, PGC-1α, and SOD in the heart tissue of healthy male rats. However, HIIT stimulates SOD gene expression more than MICT.
موضوع مقاله:
عمومى
دریافت: 1402/3/31 | پذیرش: 1402/6/3 | انتشار: 1402/7/9
دریافت: 1402/3/31 | پذیرش: 1402/6/3 | انتشار: 1402/7/9
| بازنشر اطلاعات | |
 |
این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است. |
