Izadpanah qeshmi F, Javadpour S, MalekZadeh K, Tamadoni Jahromi S, Rahimzadeh M. Isolation and identification of L-asparaginase from actinomycetes of Persian Gulf. Hormozgan Medical Journal 2014; 18 (2)
URL:
http://hmj.hums.ac.ir/article-1-1690-en.html
Abstract: (48 Views)
Introduction: L-asparaginase is an anti-neoblastic agent used in the chemotherapy of lymphoblastic leukemia. Although this enzyme is widely distributed among microorganisms, plants and animals, microorganisms have proved to be a better alternative, thus facilitating its large-scale production. Actinomycetes are filamentous, Gram-positive bacteria widely distributed in the marine environment. The aim of this study was isolation and molecular identification of L-asparaginase producing actinomycetes isolated from Persian Gulf. Methods: In this experimental study, 60 samples were collected from Persian Gulf in Hormozgan, Iran. All colonieses were screened for L-asparaginase activity with modified M9 medium. Lasparaginase activity was measured by colorimertic method. The strain with high L-asparaginase activity were selected and identified by nucleotide sequencing of 16S rRNA gene. Results: Thirty-two colonies of actinomycetes were obtained from sediment and seawater samples, among which 23 isolates were L-asparaginase producers. The strain PG08 with 37×10-2 IU activity showed the highest activity. Based on the analysis 16S rRNA gene sequences of this strain was showed maximum similarity to Streptomyces spp.OS1-33. Conclusion: Actinomycetes isolated from Persian Gulf may be potential source of enzyme Lasparaginase, Streptomyces spp. PG08 with high productivity L-asparaginase can be used for chemotherapy of acute lymphoblastic leukemia.
Type of Study:
Research |
Subject:
General Received: 2024/02/11