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Hormozgan medical journal. 2021;25(1): 19-23.
doi: 10.5812/hmj.102953
  Abstract View: 67
  PDF Download: 61

Research Article

Total Phenolic and Flavonoid Content and Antibacterial Properties of Polygonatum orientale Desf and Tiliadasystyla

Parichehr Hanachi 1* ORCID logo, Roshanak Zarringhalami 1 ORCID logo, Ertugrul Kaya 2 ORCID logo

1 Department of Biotechnology, Faculty of Biological Science, Alzahra University, Tehran, Iran
2 Medical Pharmacology, Faculty of Medicine, Duzce University, Düzce, Turkey
*Correspondence to Parichehr Hanachi, Email: p.hanachi@alzahra. ac.ir

Abstract

Background: Secondary metabolites of plants such as phenol and flavonoids have an extreme potential
for neutralizing free radicals. The antioxidant and cytotoxic activities of plants are related to phenolic
or flavonoids compounds. The occurrence of drug resistance to antimicrobial drugs has led to use of
medicinal herbs in treatment of infections. Antibiotic resistant of Staphylococcus aureus has become a
major problem in the treatment of diseases.
Objectives: The aim of this study was determination of total phenolic content (TPC) and total flavonoids
content (TFC) of Polygonatum orientale Desf and Tilia dasystyla and evaluation of their anti-bacterial
effects on Staphylococcus aureus bacterium. Investigation of TPC of P. orientale Desf and T. dasystyla has
not been reported before.
Methods: Total phenolic and flavonoid content of P. orientale Desf and T. dasystyla extracts were
determined using colorimetric methods of Folin-Ciocalteu and aluminum chloride. Antimicrobial activities
of the extracts were evaluated by microdilution broth and disc diffusion methods to determine minimum
inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values.
Results: The results showed that TFC of P. orientale Desf with the value of 7.9 ± 0.040 mg/g DW extracted
with diluted water solvent and boiling method and TPCs of T. dasystyla with the value of 62.13 ± 0.073
mg/g DW extracted with methanol solvent and boiling method were the highest amount. Methanol extract
of P. orientale Desf had more antibacterial activities with the MBC and MIC values of 0.140 mg/mL and
8±0.4 mm zone of inhibition.
Conclusion: Tilia dasystyla and P. orientale Desf contain bioactive compounds such as phenolic and
flavonoids that can be used as promising option in pharmacognostical studies for treatment of S. aureus
infections.
Keywords: Antibacterial, Flavonoid, Phenol, Polygonatum orientale Desf, Tilia dasystyla
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Submitted: 25 Mar 2020
Accepted: 06 Oct 2020
ePublished: 02 Dec 2020
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