Hormozgan Medical Journal
مجله پزشکی هرمزگان
Hormozgan Medical Journal
Medical Sciences
http://hmj.hums.ac.ir
1
admin
2423-3528
2423-3536
doi
en
jalali
1393
3
1
gregorian
2014
6
1
18
2
online
1
fulltext
en
Isolation and identification of L-asparaginase from actinomycetes of Persian Gulf
Isolation and identification of L-asparaginase from actinomycetes of Persian Gulf
عمومى
General
پژوهشي
Research
Introduction: L-asparaginase is an anti-neoblastic agent used in the chemotherapy of lymphoblastic leukemia. Although this enzyme is widely distributed among microorganisms, plants and animals, microorganisms have proved to be a better alternative, thus facilitating its large-scale production. Actinomycetes are filamentous, Gram-positive bacteria widely distributed in the marine environment. The aim of this study was isolation and molecular identification of L-asparaginase producing actinomycetes isolated from Persian Gulf. Methods: In this experimental study, 60 samples were collected from Persian Gulf in Hormozgan, Iran. All colonieses were screened for L-asparaginase activity with modified M9 medium. Lasparaginase activity was measured by colorimertic method. The strain with high L-asparaginase activity were selected and identified by nucleotide sequencing of 16S rRNA gene. Results: Thirty-two colonies of actinomycetes were obtained from sediment and seawater samples, among which 23 isolates were L-asparaginase producers. The strain PG08 with 37×10-2 IU activity showed the highest activity. Based on the analysis 16S rRNA gene sequences of this strain was showed maximum similarity to Streptomyces spp.OS1-33. Conclusion: Actinomycetes isolated from Persian Gulf may be potential source of enzyme Lasparaginase, Streptomyces spp. PG08 with high productivity L-asparaginase can be used for chemotherapy of acute lymphoblastic leukemia.
Introduction: L-asparaginase is an anti-neoblastic agent used in the chemotherapy of lymphoblastic leukemia. Although this enzyme is widely distributed among microorganisms, plants and animals, microorganisms have proved to be a better alternative, thus facilitating its large-scale production. Actinomycetes are filamentous, Gram-positive bacteria widely distributed in the marine environment. The aim of this study was isolation and molecular identification of L-asparaginase producing actinomycetes isolated from Persian Gulf. Methods: In this experimental study, 60 samples were collected from Persian Gulf in Hormozgan, Iran. All colonieses were screened for L-asparaginase activity with modified M9 medium. Lasparaginase activity was measured by colorimertic method. The strain with high L-asparaginase activity were selected and identified by nucleotide sequencing of 16S rRNA gene. Results: Thirty-two colonies of actinomycetes were obtained from sediment and seawater samples, among which 23 isolates were L-asparaginase producers. The strain PG08 with 37×10-2 IU activity showed the highest activity. Based on the analysis 16S rRNA gene sequences of this strain was showed maximum similarity to Streptomyces spp.OS1-33. Conclusion: Actinomycetes isolated from Persian Gulf may be potential source of enzyme Lasparaginase, Streptomyces spp. PG08 with high productivity L-asparaginase can be used for chemotherapy of acute lymphoblastic leukemia.
Asparaginase,Actinomycetes,16S rRNA,Persian Gulf,
Asparaginase,Actinomycetes,16S rRNA,Persian Gulf,
0
0
http://hmj.hums.ac.ir/browse.php?a_code=A-10-2-1690&slc_lang=en&sid=1
F.
Izadpanah qeshmi
F.
Izadpanah qeshmi
No
S.
Javadpour
S.
Javadpour
No
K.
MalekZadeh
K.
MalekZadeh
No
S.
Tamadoni Jahromi
S.
Tamadoni Jahromi
No
M.
Rahimzadeh
M.
Rahimzadeh
No