Volume 17, Issue 2 (5-2013)                   hmj 2013, 17(2): 113-120 | Back to browse issues page

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Khanifar J, Ahmadi A, Ghoorchian H, Haji Hosseini R, Sheikhan M, Haghirosadat F. Comparison of Essential and Non Essential Amino Acids in the Microbial Protein of Pleurotus Florida from the Lignocellulosic Wastes. hmj. 2013; 17 (2) :113-120
URL: http://hmj.hums.ac.ir/article-1-927-en.html
MSc of Biochemistry
Abstract:   (3439 Views)
Introduction: Cereal straws contain Cellulose, Hemicelluloses and Lignin and are most available renewable biopolymers. White rot fungi is used to convert these wastes into microbial protein. Pleurotus Florida are having the most delignification ability amongst other micro-organisms. We determined the amounts of protein, essential and non essential amino acids of the produced microbial protein from the wheat straw. Methods: Wheat straw was pretreated with NaOH 2% at 1000C temperature on autoclave condition. Then it was inoculated with Pleurotus Florida which provided by Mandel's media with 0.3 g/lit Urea and incubated for 4 weeks under room temperature. Protein concentration of microbial protein was determined. The extracted protein partly hydrolyzed with HCl 6 Normal and the other part hydrolyzed with Ba(OH)2 4 Normal for 48 hours under 1100C temperature condition and its amino acids analyzed by using A-200 Amino Nova analyzer. Results: The amount of protein extracted after 4 full test runs were 62.8 % per 100 g of dried microbial protein and the profile concentration of Non_essential and Essential amino acids was: Aspartic acid=5.22, Serine=3.6, Glutamic acid=6.38, Prolin=3.2, Glycine=4.21, Alanine=6.23, Cycteine=1.18, Tyrosine=2.61 Threonine=0.6, Valine=6.6, Methionine=2.1, Isoleucine=7.3, Leucine=6.8, Phenylalanine=4.3, Histidine=19.8, Lysine=9.5, Arginine=8.3, Tryptophan=0.96 g/100g of extracted protein. The ratio of essential amino acids to the total amino acids was 65.6%. Conclusion: Eessential and non essential amino acids obtained are indicative of its high quality. In the microbial protein produced by our study, considerable amounts of amino acids such as Lysine, Histidine, Lucine, Isolucine, Alanine, Arginine and Tryptophan were detected and as such it can be a proper replacement for the current available animal feed in the market.
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Type of Study: Research | Subject: General
Received: 2013/05/26 | Accepted: 2013/08/26 | Published: 2013/08/26

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