Volume 19, Number 3 (9-2015)                   hmj 2015, 19(3): 172-179 | Back to browse issues page


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Ahmadvand H, Amiri H, Dalvand H. Antioxidant properties of hydro-alcoholic extract and extract of nepeta crispa in Lorestan province. hmj. 2015; 19 (3) :172-179
URL: http://hmj.hums.ac.ir/article-1-1489-en.html

Department of Biochemistry
Abstract:   (1519 Views)

Introduction: Antioxidants protect body against the oxidative stress caused by free radicals. This study aims at comparing the antioxidant properties of hydro-alcoholic extract and extract of Nepeta crispa (from its shoot part).

Methods: The study was carried out in Lorestan University of Medical Sciences in 2012. Both hydro-alcoholic extract and essence of Nepeta crispa were prepared and antioxidant properties of samples were assessed using different methods. The ability to inhibit free radicals production was measured through 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method. Total antioxidant capacity was measured using Phosphomolybdate Method. Likewise, phenol and flavonoid compounds of samples were measured using folin-ciocalteu Zishen method.

Results: The results showed that the total antioxidant capacity of hydro-alcoholic extract and essence of Nepeta crispa were equal to 0.23±0.12 and 0.82±0.40 Nmol ascorbic acid per gram extract or essence. The phenol content of hydro-alcoholic extract and essence of Nepeta crispa were 254.63±15.75 and 6.94±1.37 mg gallic acid per gram extract or essence, respectively. Flavonoid content of hydro-alcoholic extract and essence of Nepeta crispa were equal to 5.53±0.34 and 0.36±0.06 mg quercetin per gram extract or essence, respectively. IC50 value (concentration that will inhibit 50% of free radicals production), hydro-alcoholic extract, essence of plant and Butylated hydroxytoluene (BHT), as positive control, were equal to 2.09±0.11, 6.05, and 3.88±1 μg/ml, respectively.

Conclusion: The results showed that the hydro-alcoholic extract has better antioxidant properties in contrast to essence of Nepeta crispa. As a result, hydro-alcoholic extract of Nepeta crispa is a good and available source of antioxidant compounds which can be used, if useful, in food, pharmaceutical and industrial products.

Keywords: Essential Oil – Antioxidants – Nepeta Crispa
Citation: Ahmadvand H, Amiri H, Dalvand H. Antioxidant properties of hydro-alcoholic extract and extract of nepeta crispa in Lorestan province. Hormozgan Medical Journal 2015;19(3):172-179.
Full-Text [PDF 393 kb]   (497 Downloads)    
Type of Study: Research | Subject: General
Received: 2015/10/31 | Accepted: 2015/10/31 | Published: 2015/10/31

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