Volume 16, Number 6 (1-2013)                   hmj 2013, 16(6): 429-435 | Back to browse issues page


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Monocyte isolation using serum dextrose 5% with autologous plasma. hmj. 2013; 16 (6) :429-435
URL: http://hmj.hums.ac.ir/article-1-838-en.html

Abstract:   (5412 Views)
Introduction: Mammalian cells culture is an important method for producing recombinant proteins, monoclonal antibody, and hormones application. One of the common methods in the monocyte isolation is using flask adherent cell culture in RPMI-1640 medium. We aimed, in this study, cell culture medium using dextrose 5% with autologous plasma to produce inexpensive medium for isolation of monocytes. Methods: In this experimental study, we isolated peripheral blood mononuclear cells from 40 healthy volunteers, the isolated cells were cultured in RPMI 1640 medium containing 10% fetal calf serum and in the media supplemented serum dexterous 5%containing autologous plasma under 37°C and 5% CO2 in a cell incubator. Then, count, viability and morphological cells were surveyed by trypan blue and invert microscope. Results: We isolated 1.8- 2× 106 cells /ml of RPMI culture media and 2- 2.2×106 /ml macrophage of serum dexterous 5% containing autologous plasma. There was not a significant difference between two methods (P>0.05). Also, we did not see any morphological variation in two culture media. Conclusion: Our results showed that dextrose 5% can be used in monocyte isolation from peripheral blood mononuclear cells
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Type of Study: Research | Subject: General
Received: 2013/01/15

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