Volume 20, Issue 1 (4-2016)                   hmj 2016, 20(1): 33-38 | Back to browse issues page

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Haniyeh R, Hamzeh A, Ahmad G. Antioxidant properties and Flavonoids-Phenolic content of citrullus colocynthis (L.) schrad growing in Khuzestan, Iran. hmj. 2016; 20 (1) :33-38
URL: http://hmj.hums.ac.ir/article-1-1567-en.html
Department of Plant Physiology
Abstract:   (1653 Views)

Introduction: Free radicals cause several human diseases. Antioxidants decrease the risk of cardiovascular diseases and heart attack, and prevent the development of cancers through neutralization of free radicals. This study set out to determine the antioxidative properties of Citrullus colocynthis growing in Khuzestan. This plant is traditionally used for the treatment of type II diabetes.

Methods: In this study, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and beta-carotene linoleic acid assays were used for the evaluation of antioxidative properties. In addition, the measurement of flavonoids and phenolic compounds was done based on the Gallic acid and quercetin reagents.


Hamzeh Amiri, PhD.

Biology Department Faculty of Science, Lorestan University.

Khorramabad, Iran

Tel:+98 9166634064



Results: In the DPPH assay, the maximum and minimum scavenging activity of free radicals were found in the extractions of stem (IC50=12.4μg/ml) and leaves (IC50=5.46 μg/ml), respectively. With respect to the beta-carotene linoleic assay, the maximum and minimum antioxidative activities were observed in the extractions of leaves (79.78%) and fruits (47.08%), respectively. Moreover, the maximum content of flavonoids and phenolic compound were 22.15μg/ml in stem, and 4.09μg/ml in fruit.

Conclusion: The methanol extracts from different organs of citrullus colocynthis (C. colocynthis) showed antioxidative activities at different levels. Therefore, they can be used as antioxidants in food industries and adjuvant treatments.

Keywords: Citrullus Colocynthis, Antioxidative, Flavonoids
Citation: Rashedi H, Amiri H, Gharezi A. Antioxidant properties and Flavonoids-Phenolic content of citrullus colocynthis (L.) schrad growing in Khuzestan, Iran. Hormozgan Medical Journal 2016;20(1):36-41.
Full-Text [PDF 1012 kb]   (398 Downloads)    
Type of Study: Research | Subject: General
Received: 2016/07/19 | Accepted: 2016/07/19 | Published: 2016/07/19

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